Fig. 1

Transcriptome alterations in high glucose–treated HMrSV5 cells. a Volcano plots illustrating the DEGs detected by RNA-seq. The data were filtered by a P value < 0.05 and an absolute fold change > 2. b, c KEGG enrichment (b) or hallmark gene set enrichment (c) of upregulated DEGs and downregulated DEGs. Up/Down: upregulated or downregulated genes in high glucose-treated HMrSV5 cells. d, e Heatmap showing dysregulated genes associated with hallmark of epithelial mesenchymal transition (d) or the TGF-beta signaling pathway. f, g Gene Set Enrichment Analysis (GSEA) shows that the TGF-beta signaling pathway (f) and Epithelial mesenchymal transition hallmark gene set (g)  were enriched in high-glucose treated HMrSV5 cells