Fig. 4

Cellular interaction analysis. A Volcano plot showing most significant cell–cell interactions enriched in patient response groups. Interactions were defined in Scimap by proximity of cell pairs within 20um normalised to the number of those cell types within each sample. These proximity densities were compared by T test between response groups, and unadjusted p values shown. B Violin plot of Treg: monocyte interaction frequency. p = 0.009. C Representative field of view of enriched Treg: monocyte interactions. D Violin plot of macrophage: HLADR⁺ tumour cell interaction frequency. p = 0.01 E Representative field of view of enriched macrophage: HLADR⁺ tumour cell interactions. F Violin plot of Treg: CD8⁺ cell interaction frequency. p = 0.009 G Representative field of view of enriched Treg: CD8⁺ interactions. H ROC curve indicating AUC of generalised linear model for binary classification of patient response by most significant interactions as well as when combined into multivariate model. Significant results p < 0.05 in volcano shown in red. Not adjusted for multiple testing