Fig. 3

Cell proportion analysis within tissue compartments. A, F Representative TMA cores with tumour (red) and stroma (green) compartments. B, C Composition of the stromal compartment appeared similar in IO response groups. D Variance in patient stroma was largely driven by proportions of CD4⁺, B cells, blood vessels and macrophages. E Treg cells and monocytes were slightly elevated in non-responding patients. G, H Composition of the tumour compartment appeared similar in IO response groups. I Variance in tumour regions was driven by tumour cell CD44 and HLADR positivity, as well as levels of granulocyte infiltration. J Proliferating tumour cells were more abundant in non-responding patients. K) Tumour regions were expanded by 30um into the stroma to define a tumour margin (White). L, M Composition of the tumour margin appeared consistent between patient response groups. N Levels of CD4⁺, macrophages and monocyte cells were major components of variance in tumour interface. O Monocytes were enriched in tumour margin regions of non-responding patients. Significant results p < 0.05 in volcano shown in red. Not adjusted for multiple testing