Fig. 5

Adding TH to a cold preservation solution prevents IR-induced inflammatory gene expression. Cells were treated with or without T3 (2.5 μM) or T4 (25 μM) during 6 or 18 h cold preservation, followed by 4 h warm reperfusion. Gene expression was assessed with NanoString and expressed as a ratio relative to the housekeeping gene ABCF1. A T3 or T4 reduced the numbers of differentially expressed (DE) genes. B–F. T3 or T4 prevented IR-induced expression of IL-6, NFATC2, CXCL1/2, CXCL2, and IL-8 (compared to untreated group after 18 h cold preservation). N = 3 samples/group, one-way ANOVA followed by Tukey post-hoc test. **p < 0.01