Fig. 3

Adding TH to a cold preservation solution prevents cell injury during warm reperfusion. A Cell morphology was assessed with phase contrast microscopy. T3 (2.5 µM) or T4 (25 µM) was added to Perfadex® solution during cold preservation for 18 h, followed by warm reperfusion for 2 h. Perfadex® solution alone or with A1AT (2 mg/mL) was used for comparison. B T3 (2.5 μM) or T4 (25 μM) treatment elevated mitochondrial membrane potential. The specificity of the test was verified with FCCP treatment, which is an uncoupler of oxidative phosphorylation. C T3 or T4 treatment reduced mitochondrial superoxide production dose-dependently. N = 4 samples/group, one-way ANOVA followed by Tukey post-hoc test. *p < 0.05; **p < 0.01; ***p < 0.001, compared to no drug control