Fig. 2

Adding TH to a cold preservation solution protects cell viability and inhibits caspase activation during warm reperfusion. A T3 or T4 dose-dependently improved cell viability after 18 h cold preservation and 4 h reperfusion (*p < 0.05 compared with no treatment control). B Role of caspase 3, 8, and 9 in mediating apoptosis. C T3 (2.5 μM) or T4 (25 μM) treatment prevented activation of caspase 3, 8, and 9 after 18 h CIT and 2 h reperfusion. N = 4 samples/group, one-way ANOVA followed by Tukey post-hoc test. *p < 0.05; **p < 0.01