Fig. 1

TBC1D5 acted as diagnostic and prognostic biomarkers in ccRCC connecting HIF-2α and autophagy. A Heatmap of the expression profiles of 21 autophagy-related different expressed genes (DEGs) in the TCGA-KIRC. B Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of DEGs that significantly correlated with HIF-2α indicated these DEGs enriched in multiple autophagy-related pathways. C Venn diagram for DEGs showing autophagy and HIF-2α related genes based on TCGA-KIRC, sequencing data of HIF-2α knockdown RCC cell line, sequencing data of ccRCC tissue, and three independent Gene Expression Omnibus (GEO) datasets. D Kaplan–Meier curves of disease-free survival (DFS) and ROC curves for two selected genes, TBC1D5 and AFT88. E The mRNA levels of TBC1D5 in paired or unpaired tumor tissue and paracancer tissue. F–H Patients with relatively high-risk T, M, and tumor stages have lower expression of TBC1D5. I The patients with Clear cell renal cell carcinoma (ccRCC) of higher histologic grade also experience a lower expression of TBC1D5. J, K Deceased patients and progressed patients have a lower expression of TBC1D5. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001