Fig. 3

Morn2^–/– sperm exhibit bent heads and significantly decreased motility. A Hematoxylin and eosin staining of epididymal sperm smears in Morn2^+/– and Morn2^–/– mice. The test was replicated three times using distinct biological samples. The scale bar is 20 μm. B The percentage of epididymal sperm with abnormal phenotypes in Morn2^+/– (11.28 ± 1.41%) and Morn2^–/– (55.21 ± 6.32%) mice. At least 200 spermatozoa were counted in each sample. The data are shown as the mean ± SEM of three independent experiments using distinct biological samples. Each data point represents the percentage of sperm with abnormal phenotypes per sample, *** = P < 0.001 by Student’s t-tests. C The percentage of epididymal sperm with bent heads in Morn2^+/– (3.75 ± 1.78%) and Morn2^–/– (44.59 ± 4.94%) mice. At least 200 spermatozoa were counted in each sample. The data are shown as the mean ± SEM of three independent experiments using distinct biological samples. Each data point represents the percentage of sperm with bent heads per sample, *** = P < 0.001 by Student’s t-tests. D The VAP of spermatozoa in Morn2^+/– (45.26 ± 1.42 μm/s) and Morn2^–/– (13.34 ± 5.21 μm/s) mice (n = 3). At least 200 spermatozoa were counted in each sample. The data are shown as the mean ± SEM of three independent experiments using distinct biological samples. Each data point represents the VAP of spermatozoa in an individual, **** = P < 0.0001 by Student’s t-tests. E The VSL of spermatozoa in Morn2^+/– (39.52 ± 2.08 μm/s) and Morn2^–/– (10.49 ± 3.78 μm/s) mice (n = 3). At least 200 spermatozoa were counted in each sample. The data are shown as the mean ± SEM of three independent experiments using distinct biological samples. Each data point represents the VSL of spermatozoa in an individual, **** = P < 0.0001 by Student’s t-tests. F The VCL of spermatozoa in Morn2^+/– (75.26 ± 3.76 μm/s) and Morn2^–/– (29.81 ± 13.11 μm/s) mice (n = 3). At least 200 spermatozoa were counted in each sample. The data are shown as the mean ± SEM of three independent experiments using distinct biological samples. Each data point represents the VCL of spermatozoa in an individual, **** = P < 0.0001 by Student’s t-tests. G The percentage of motile spermatozoa in Morn2^+/– (71.97 ± 2.55%) and Morn2^–/– (6.93 ± 3.44%) mice. At least 200 spermatozoa were counted in each sample. The data are shown as the mean ± SEM of three independent experiments using distinct biological samples. Each data point represents the percentage of motile spermatozoa in an individual, **** = P < 0.0001 by Student’s t-tests. H The percentage of progressive motile spermatozoa in Morn2^+/– (7.97 ± 2.16%) and Morn2^–/– (0.07 ± 0.15%) mice. At least 200 spermatozoa were counted in each sample. The data are shown as the mean ± SEM of three independent experiments using distinct biological samples. Each data point represents the percentage of progressive motile spermatozoa in an individual, **** = P < 0.0001 by Student’s t-tests. I The IVF rate with cumulus-intact oocytes of Morn2^+/– (83.14 ± 10.63%) and Morn2^–/– (41.18 ± 22.19%) mice. The experiment involved 3 Morn2^+/– or Morn2^+/+ male mice, 3 Morn2^–/– male mice, and 30 WT female mice, with a total of 446 oocytes included in the analysis. The data are shown as the mean ± SEM of three independent experiments. Each data point represents the fertilization rate of a male individual, *P < 0.05 by Student’s t-tests. J The in vivo fertilization rate of Morn2^+/– (60.88 ± 19.81%) and Morn2^–/– (0%) mice. The experiment involved 3 Morn2^+/– or Morn2^+/+ male mice, 3 Morn2^–/– male mice, and 12 WT female mice. The data are shown as the mean ± SEM of three independent experiments. Each data point represents the fertilization rate of a male individual, ** = P < 0.01 by Student’s t-tests