Fig. 6

OGFOD1 knockdown activates UPR and protects cells against I/R injury. A The mRNA level of Ogfod1 in HT-22 cells transfected with Con-siRNA and Ogfod1-siRNA for 36 hours previously. Con-siRNA vs Ogfod1-siRNA: ***p < 0.001 (student’s t-test. n = 3). B Representative western blots of OGFOD1, sATF6, sXBP1, p-eIF2ɑ, Beclin-1, LC3-II and p62/SQSTM1 in HT-22 cells. Cells were transfected with Con-siRNA or Ogfod1-siRNA for 48 hours and underwent OGD (3 hours)/R (6 hours). C The quantitative results of OGFOD1 in HT-22 cells transfected with con-siRNA or Ogfod1-siRNA for 48 hours and subjected to OGD/R. Data are presented as the mean ± SEM, Con-siRNA vs Ogfod1-siRNA: **p < 0.01, Ogfod1-siRNA vs Ogfod1-siRNA + OGD/R: ##p < 0.01, Ogfod1-siRNA+OGD/R vs Con-siRNA+OGD/R: †††p < 0.001 (one-way ANOVA followed by Dunnett’s post-hoc test, n = 3). D The cell viability of HT-22 cells was measured by the absorbance of formazan products following OGD/R. Cells were transfected with Con-siRNA or Ogfod1-siRNA for 48 hours and underwent OGD (3 hours)/R (6 hours). Results are presented as mean ± SEM from four independent experiments, Con-siRNA vs Ogfod1-siRNA or Ogfod1-siRNA+OGD/R: ***p < 0.001, Ogfod1-siRNA vs Ogfod1-siRNA+OGD/R: ###p < 0.001, Ogfod1-siRNA+OGD/R vs Con-siRNA+OGD/R: †††p < 0.001 (one-way ANOVA followed by Dunnett’s post-hoc test). E and F The quantitative results of sATF6, sXBP1, p-eIF2ɑ, Beclin-1, LC3-II and p62/SQSTM1 in HT-22 cells transfected with Con-siRNA or Ogfod1-siRNA for 48 hours and subjected to OGD/R. Data are presented as the mean ± SEM, Con-siRNA vs other groups: ***p < 0.001, **p < 0.01, *p < 0.05. Ogfod1-siRNA+OGD/R vs Ogfod1-siRNA or Con-siRNA+OGD/R: ###p < 0.001, ##p < 0.01, #p < 0.05 (one-way ANOVA followed by Dunnett’s post-hoc test, n = 3)