Fig. 1

FG4592 alleviates injury and improves the recovery of sensorimotor defects after transient ischemia. A Representative TTC staining image of brain sections from mice after 24 h of tMCAO. Layer thickness = 1 mm, scar bar = 1 cm. B Infarct volume 24 h after tMCAO in the vehicle (n = 10), FG4592 (2.5 mg/kg, n = 10), FG4592 (5 mg/kg, n = 10), FG4592 (10 mg/kg, n = 10) and Edaravone (3 mg/kg, twice after reperfusion, n = 10) groups. Data are presented as mean ± SEM. Vehicle vs other groups: **p < 0.01 (one-way ANOVA followed by Dunnett’s post-hoc test). C mNSS score of mice after 24h of tMCAO. Data are presented as mean ± SEM. Vehicle vs other groups: *p < 0.05 (non-parametric Kruskal-Wallis test followed by Dunn’s post-hoc). D-G The sensorimotor functions of mice were analyzed by mNSS score, cylinder test, foot fault task and adhesive removal test up to 28 days after tMCAO. Data are presented as mean ± SEM. FG4592 (n = 11) vs vehicle (n = 12): ***p < 0.001, **p < 0.01, *p < 0.05. Edaravone (n = 11) vs vehicle: ###p < 0.001, ##p < 0.01, #p < 0.05 (two-way repeated-measures ANOVA followed by Holm-Sidak post-hoc multiple-comparison tests). H The representative crystal violet staining images correspond to coronal brain sections from mice after 28 days of tMCAO. Five mice were randomly selected from each group after the behaviour tests. Scar bar = 1cm. I Measurement of the atrophy volume of the infarct hemisphere. Vehicle vs FG4592: *p < 0.05, ns: no significance (one-way ANOVA followed by Dunnett’s post-hoc test, n = 5). J The representative TTC staining images of mice administrated with FG4592 at different time points after tMCAO. Layer thickness = 1 mm, scar bar = 1 cm. K and L The infarct volumes (one-way ANOVA followed by Dunnett’s post-hoc test) and mNSS scores (non-parametric Kruskal-Wallis test followed by Dunn’s post-hoc) of the vehicle (n = 12), 0-hour (n = 12), 3 hours (n = 12), 6 hours (n = 12) and 12 hours (n = 12) groups. Data are presented as mean ± SEM. vehicle vs other groups: ***p < 0.001, **p < 0.01, *p < 0.05, ns: no significance