Fig. 5

The in vitro uptake and effect of DSPE-PEG-CMP-miR302-EXO on gene expression in H9C2 cells. A Fluorescence images indicated internalization of BMSCs-EVs and DSPE-PEG-CMP-miR302-EXO in H9C2 cells. Scale bar = 50 μm. B Quantitative polymerase chain reaction (qRT-PCR) indicated upregulation of miR302 level in H9C2 cells by DSPE-PEG-CMP-miR302-EXO. Relative values were presented as fold change over control. C The MTT assay showed increased proliferation ratio of H9C2 cells by DSPE-PEG-CMP-miR302-EXO. D–F Western blot analysis of Ki67 and Yap levels displayed increased levels of these proteins in cardiomyocytes after co-incubation with DSPE-PEG-CMP-miR302-EXO. GAPDH served as the internal reference. Relative values were presented as fold change over control. a: Control; b: Model; c: DSPE-PEG-CMP; d: DSPE-PEG-CMP-EXO; e: DSPE-PEG-CMP-miR302-EXO; f: miR302. B, E, F ^▲▲p < 0.01 vs control; *p < 0.05, **p < 0.01 vs Model; ^##p < 0.01 vs DSPE-PEG-CMP-EXO; ^&p < 0.05, ^&&p < 0.01 vs miR302. C *p < 0.05, **p < 0.01 vs DSPE-PEG-CMP; ^##p < 0.01 vs DSPE-PEG-CMP-EXO; ^&p < 0.05, ^&&p < 0.01 vs miR302