Fig. 5

GP73 knockout blocks tumor development of HBV; Pten^−/− mice. A Immunoblotting analysis of 4-month-old WT, HBV, Pten^−/−, and HBV; Pten^−/− livers (upper panel) and tumors and adjacent liver tissues from 9-month-old HBV; Pten^−/− mice (lower panel). T: tumor, N: adjacent tissues. B–D 9-month-old HBV; Pten^−/− and HBV; Pten^−/−; Gp73^−/− mice. B The ratio of liver weight to body weight of mice (n = 15). C Representative images of livers. D Number (> 3 mm) and maximal size of liver tumors (n = 15). E Immunoblotting analysis of GP73 and cleaved-caspase 3 of 4-month-old HBV; Pten^−/− and HBV; Pten^−/−; Gp73^−/− livers. F Masson’s trichrome, CD34 and Cleaved Caspase 3 staining of 9-month-old HBV; Pten^−/− and HBV; Pten^−/−; Gp73^−/− livers. G Serum ALT and AST of 4-month-old HBV; Pten^−/− and HBV; Pten^−/−; Gp73^−/− mice (n = 10–11). H Immunoblotting analysis of 4-month-old HBV; Pten^−/− and HBV; Pten^−/−; Gp73^−/− livers. I qRT-PCR analysis of IL6 mRNA level in 4-month-old HBV; Pten^−/− and HBV; Pten^−/−; Gp73.^−/− livers (n = 10). Scale bar, 100 μm. Data were shown as mean ± SD, **p < 0.01, ***p < 0.001