Fig. 2

Senescent cell intervention impacts re-epithelialization and collagen deposition. A Schematic diagram of senolytic ABT-263 clearing senescent cells and sample collection. B Representative gross images, HE staining images, and Masson staining images showing reduced damage in the irradiated limbs after ABT-263 treatment compared to the PBS control, n = 5. C Schematic illustration of the target construction for p16^{DTR−tdTomato} mice and CRISPR gene editing. D Representative data from flow cytometry analysis of the proportion of tdTomato⁺ in single-cell suspensions of skin from young mice (2 months old), aged mice (18 months old), and aged mice after senescent cells clearance, n = 5. E Schematic diagram of using diphtheria toxin to target and clear senescent cells and sample collection. F Representative gross images showing reduced damage in the irradiated limbs after diphtheria toxin treatment compared to the PBS control. G Gross injury scores comparing the senescent cells clearance groups and the control groups, n = 3 for each group. Representative images of H HE staining, I Ki67⁺ proliferative cells immunofluorescent staining, J α-SMA⁺ myofibroblasts immunohistochemical staining and CD31⁺ K endothelial cells immunohistochemical staining comparing the senescent cells clearance groups and the control groups. L Quantitative analysis of the positive signals of Ki67, α-SMA, and CD31 between the senescent cell clearance groups and the control groups. 12 high-magnification fields were randomly selected, and the proportion of positive signal cells (Ki67) or the area of positive signals (α-SMA, CD31) were analyzed using ImageJ software. Data were presented as mean ± standard deviation. When variance was met, a t-test was used for statistical analysis between two groups, while ANOVA was used for comparisons among three or more groups. If variance wasn’t met, the Mann-Whitney U test was used. *p < 0.05, **p < 0.01, ***p < 0.001. Bars represented 100 μm