Fig. 3

A Representative TEM images of autophagosome (single black arrow) and autophagolysosome (double black arrow) in the cytoplasm of PANC-1. B, C Representative western blot images and semi-quantification analysis of p62 expression in PANC-1 cells. D–F Representative fluorescent images of p62 (red) and LC3II punctuation (green) in PANC-1 cells, and the quantification analysis of mean fluorescence intensity per cell. Nuclei were stained with DAPI (blue). *P < 0.05, **P < 0.01, ****P < 0.0001