Fig. 5
From: Hypoxia promotes metastasis by relieving miR-598-3p-restricted glycolysis in gastric cancer
miR-598-3p suppressed glycolysis in GC cells by targeting RMP and IGF1r. A, B Glucose uptake (A) and lactate production (B) in MKN45 cells transfected with the indicated plasmid. C, D ATP production (C) and ATP source (D) of BGC-823 cells transfected with the indicated plasmid. E, F ATP production (E) and ATP source (F) of MKN45 cells transfected with the indicated plasmid. G, H Western blot analysis of glycolytic enzymes in MKN45 and BGC-823 cells overexpressing miR-598-3p (G) or the sponge (H). I–L Real-time qPCR analysis of GLUT1, HK2, PKM2 and LDHA expression in MKN45 and BGC-823 cells expressing miR-598-3p or the sponge. M–P Correlation analysis between miR-598-3p and LDHA (M), GLUT1 (I), HK2 (J) and PKM2 (K) expression in 20 GC samples