Fig. 2
From: Hypoxia promotes metastasis by relieving miR-598-3p-restricted glycolysis in gastric cancer
miR-598-3p suppressed hypoxia-induced metastasis in vitro and in vivo. A–C Wound-healing assay of the indicated cells transfected with GFP or miR-598-3p in 1%O2. D, E Transwell assay of SGC-7901 and MKN45 cells transfected with GFP or miR-598-3p in hypoxia (1%O2). F Real-time PCR analysis of miR-598-3p in SGC-7901 and MKN45 cells transfected with GFP or miR-598-3p. G, H BALB/c nude mice injected via the tail vein with SGC-7901 and MKN45 cells expressing GFP or miR-598-3p, respectively. The mice were sacrificed after 4 weeks, and the white arrow depicts metastatic nodes, n ≥ 5. I, J BALB/c nude mice intraperitoneally injected with MKN45 cells expressing GFP or miR-598-3p. The mice were sacrificed after 4 weeks, and the white arrow depicts a metastatic node, n ≥ 5. K Kaplan‒Meier survival curve of BALB/c nude mice injected via the tail vein with SGC-7901 cells expressing GFP or miR-598-3p, n = 10. L Western blot analysis of SGC-7901 cells transfected with miR-598-3p under hypoxia (1%O2)