Fig.Ā 7
From: Endothelium-specific deletion of p62 causes organ fibrosis and cardiac dysfunction
Knockdown of p62 in HUVEC significantly increases the expression of FN1, LAMC2, and other EndoMT markers. A Immunofluorescence detection of FN1 expression following p62 knockdown. Scale barsā=ā50Ā Ī¼m. B Immunofluorescence detection of LAMC2 expression following p62 knockdown. Scale barsā=ā50Ā Ī¼m. C Validation of FN1 and LAMC2 expression after transfection with p62siRNA or p62 overexpression plasmid using Western blot. D Semiquantitative analysis of FN1/LAMC2 in NC group and KD group at protein level. E Semiquantitative analysis of FN1/LAMC2 in Vector group and OE group. F Validation of endothelium mesenchymal transition (EndoMT) marker expression after transfection with p62siRNA or p62 overexpression plasmid using Western blot. G Semiquantitative analysis of EndoMT markers in NC group and KD group at protein level. H Semiquantitative analysis of EndoMT markers in Vector group and OE group. *pā<ā0.05 and ** pā<ā0.01