Fig.Ā 6
From: Endothelium-specific deletion of p62 causes organ fibrosis and cardiac dysfunction
Knockdown of p62 in HUVEC increases cell apoptosis and inhibits migration. A Immunofluorescence detection of p62 localization in HUVEC. Scale barsā=ā50Ā Ī¼m. B Immunofluorescence showing validation of p62 (green) knockdown efficiency in HUVEC. DAPI dyed the nucleus blue. Scale barsā=ā50Ā Ī¼m. C. Electron microscopy image of HUVEC. Scale barsā=ā5Ā Ī¼m. D Flow cytometry analysis to detect apoptosis in HUVEC. E Quantitative analysis of apoptotic cells. F Wound healing assay to assess cell migration. ** pā<ā0.01