Fig. 6

Wnt/β-catenin pathway is hyperactivated in oxaliplatin-resistant cells. A Coomassie staining of total proteins in HCT8 and HCT8/L-OHP cells transfecting with Flag-IMPDH2 plasmids separated by SDS–PAGE. B Venn diagrams displaying the number of proteins identified in HCT8 and HCT8/L-OHP cells. C KEGG pathway enrichment analysis of proteins identified in HCT8/L-OHP cells. D and E QPCR and western blotting analysis of the activation of the Wnt/β-catenin pathways in HCT8/L-OHP cells compared to that in HCT8 cells. F and G QPCR and western blotting analysis were conducted to evaluate the suppressive effect of XAV939 on Wnt/β-catenin pathways and IMPDH2 in HCT8/L-OHP cells. H and I The cell apoptosis was analysed after XAV939 and/or oxaliplatin treatment with or without GMP compared with that of untreated cells for 24 h by flow cytometry analysis in HCT8/L-OHP cells. J Western blotting analysis of apoptotic proteins expression was performed in HCT8/L-OHP cells after XAV939 and/or oxaliplatin treatment with or without GMP. Data are presented as the mean ± SD of three independent experiments. **p < 0.01, and ***p < 0.001