Fig. 5

IMPDH2 regulates cell apoptosis of CRC in response to oxaliplatin. A and B Overexpression of IMPDH2 was confirmed at the mRNA and protein level in HCT8 cells by qPCR and western blotting. C Comparison of apoptosis induction between vector and IMPDH2-overexpression cells in HCT8 after oxaliplatin treatment was carried out by flow cytometry analysis. Bar charts show the percentage of apoptotic and surviving cells, respectively. D and E The mRNA and protein expression levels of stable knockdown IMPDH2 in HCT8/L-OHP cells were quantified by qPCR and western blotting analysis. F Flow cytometry analysis was performed to analyse the apoptotic rate in shNC and shIMPDH2 HCT8/L-OHP cells after oxaliplatin treatment. G Western blotting analysis of apoptotic proteins expression was carried out between vector and IMPDH2-overexpression HCT8 cells after oxaliplatin treatment. H Western blotting analysis was performed to detect the expression of apoptotic proteins in shNC or IMPDH2-knockdown HCT8/L-OHP cells after oxaliplatin treatment. I The apoptotic rate was analysed after GMP and/or oxaliplatin treatment compared with that of untreated cells for 24 h by flow cytometry analysis in HCT8 cells. J The apoptotic rate was analysed after MPA and/or oxaliplatin treatment compared with that of untreated cells for 24 h by flow cytosmetry analysis in HCT8/L-OHP cells. Data are presented as the mean ± SD of three independent experiments. **p < 0.01, and ***p < 0.001