Fig. 4

IMPDH2 expression is associated with CRC cells sensitivity to oxaliplatin. A and B The endogenous expression of IMPDH2 in four CRC cell lines, SW620, RKO, HCT116 and HCT8, was determined by qPCR and western blotting analysis. The mRNA and protein expression of IMPDH2 in HCT8 C and D and SW620 E and F in response to oxaliplatin treatment were determined by qPCR and western blotting analysis. G Flow cytometric analysis was performed to analyse the apoptotic rate in HCT8 and SW620 cells treated with increasing concentrations of oxaliplatin at 24 h. The histograms show the percentage of apoptosis H and surviving I cells, respectively, in both untreated and oxaliplatin-treated groups. Data are presented as the mean ± SD of three independent experiments. ns  not significant, ***p < 0.001