Fig. 1

Establishment and characterization of the acquired oxaliplatin-resistant CRC cell line HCT8/L-OHP. A CCK8 assay to assess the IC50 of oxaliplatin-resistant CRC cells HCT8/L-OHP and parental HCT8 cells after treated with increasing concentrations of oxaliplatin treatment for 48 h. B The IC50 of oxaliplatin to HCT8 and HCT8/L-OHP cells were calculated from the inhibition curves. C P-gp protein expression in HCT8 and HCT8/L-OHP cells was determined by western blotting analysis. D Flow cytometry analysis measures the apoptosis in HCT8 and HCT8/L-OHP cells at 24 h after oxaliplatin treatment. Bar charts show the percentage of apoptosis E and surviving F cells, respectively, in both untreated and oxaliplatin-treated groups. Data are presented as the mean ± SD of three independent experiments. ***p < 0.001