Fig. 8

Representative 2-dimensional dot plot graphics of the flow cytometry data for the immune and inflammatory infiltrate in BAL fluid collected from the three hamster groups (C, MCT and MCT-siRNA AP-1). Immune and inflammatory cells were incubated with different antibodies for markers specific to these cell types as follow: a CD3e-FITC for T cells, b CD4-PE for T helper cells, c CD8-APC for T cytotoxic cells, d MHC-II-PE for proinflammatory macrophages type M1, e CD11c-AF647 for dendritic cells, macrophages, neutrophils, f Singlec-1-AF647 for alveolar macrophages. Unstained cells were distributed in the left quadrant and stained cells were distributed in the right quadrant (measurements include the percentage of cells within the gate). A total of 10.000 events counted for each marker