Fig. 6

A Representative bright-field microscopy images depicting from top to bottom H&E-stained sections with whole heart, right ventricle, lung and pulmonary artery taken from healthy hamsters (C group) and hamsters after 12 weeks of MCT in the absence or presence of siRNA AP-1 injections given for 10 weeks (MCT group and MCT-siRNA AP-1 group). Four different microscopic fields for each experimental point were analysed. Nuclei: blue, cytoplasm: pink, muscle fibres: deep red. B Representative bright-field microscopy images depicting Picro- Sirius Red-stained sections with right ventricle and lung taken from healthy hamsters (C group) and hamsters after 12 weeks of MCT in the absence or presence of siRNA AP-1 injections given for 10 weeks (MCT group and MCT-siRNA AP-1 group). Four different microscopic fields for each experimental point were analysed. Nuclei, cytoplasm and muscular fibers are visualized in yellow. Collagen fibers are manifested in a striking manner as vivid orange-red bands. Scale bar indicate: 100 µm, magnification × 20