Fig. 11
Bar graph that illustrates relative expression levels of six miRNAs, miRNA-124, miRNA-145, miRNA-204, miRNA-210, miRNA-21 and miRNA-214, extracted from A pulmonary arteries and B right ventricle of the three groups of investigated animals. Total RNA was extracted and used for RT-qPCR assay. The expression of miRNA panel was validated using four tissue samples from each group and matched normal tissue samples. The miRNA expression was normalised using snRU6 as a reference gene. P-values of significant differences between the groups were calculated, and represented as ***P < 0.005, **P < 0.01, *P < 0.05 values versus control group, and ###P < 0.005, #P < 0.05 values versus MCT group (One-way ANOVA Bonferroni post-test analysis). The mean fold change in expression of the target miRNA was calculated using ∆∆Ct = ΔCt (a target sample)−ΔCt (a reference sample). For the control sample, ∆∆Ct equals 0 and 20 equals 1, therefore fold change in gene expression relative to control equals 1