Fig. 10

Representative Western blotting images of the expression levels of AP-1S3 (a), pFAK, FAK (b), pERK, ERK and β-actin/GAPDH (c) in pulmonary artery homogenates from all three experimental animal groups after 12 weeks of MCT alone or in combination with siRNA AP-1 for 10 weeks. Histograms show a quantitative representation of signalling molecules, AP-1S3 (a.1), pFAK, FAK (b.1), pERK, ERK (c.1). The data are shown as the mean ± SD of 4 independent experiments. Statistical significance represented as **P < 0.01, *P < 0.05 values versus control group and ^###P < 0.005, ^##P < 0.01, ^#P < 0.05 values versus MCT group, One-way ANOVA, Bonferroni post-test. Quantification of band intensities expressed as ratio with β-actin/GAPDH was analysed by TotalLab TL120 program. The housekeeping β-actin and GAPDH proteins are shown as loading control for protein normalization