Fig. 5From: The occurrence and development mechanisms of esophageal stricture: state of the art reviewTreatment of the artificial ulceration after esophageal ESD by transplantation of autologous oral mucosal epithelial cell sheets fabricated on temperature-responsive culture inserts. a Biopsy specimens were taken from the patient’s own oral, buccal mucosal tissue. Oral epithelial cells were isolated from the tissue by dispase I and trypsin. b The epithelial cells were seeded onto temperature-responsive culture inserts without a 3T3 feeder layer and cultured with autologous serum for 16 days at 37 °C. c Oral mucosal epithelial cell sheets were harvested by reducing the culture temperature to 20 °C. d, e Autologous oral mucosal epithelial cell sheets on a support membrane were transplanted with endoscopic forceps onto the bed of the esophageal ulceration immediately after ESD (diagrammatically displayed by us according to the work of literature [119])Back to article page