Fig. 6

In vitro validation has demonstrated that PITPNC1 governs the upregulation of CD155 via FASN in radioresistant cell lines, leading to immune evasion. A The analysis of the proportion of CD8⁺ T cells after co-culturing PBMCs with tumor cells performed by flow cytometry. B After co-culturing PBMCs with tumor cells, the flow cytometry analysis demonstrated the changes in the proportion of IFN-γ⁺ CD8⁺ T cells. C The flow cytometry analysis of the apoptosis rate of tumor cells in co-cultivation of PBMCs and tumor cells. D The alterations in the proportion of IFN-γ⁺ CD8⁺ T cells assessed by flow cytometry after co-culturing CD8⁺ T cells with tumor cells. N = 3. E The flow cytometry analysis of the apoptosis rate of tumor cells after co-culture. N = 3. Data indicate the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, by 2-tailed Student’s t test or one-way ANOVA. N = 3