Fig. 5

PITPNC1 modulated the upregulation of CD155 via FASN in radioresistant cell lines. A, B The transcriptome sequencing analysis of SW480-RR cell line after knocking down FASN. The horizontal axis of heatmaps represented the samples and the vertical axis exhibited the genes. The color gradient ranges from blue to red, and this indicated the expression levels from low to high. Box plots were plotted with sample names on the horizontal axis and log10(FPKM) on the vertical axis. C TIMER2.0 for the correlation analysis of FASN and CD155 in colorectal cancer. D qPCR fluorescence quantification analysis to assess the mRNA expression levels of FASN and CD155 after knocking down FASN. E To evaluate the expression levels of CD155 by western blotting after silencing FASN in tumor cell lines. F Immunofluorescence co-localization of CD155 and FASN proteins in tissue slides and cells. G To assess the expression levels of PITPNC1, FASN, and CD155 in tumor cell lines after individually knocking down PITPNC1 and FASN, as well as overexpressing FASN after PITPNC1 downregulation. Data indicate the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, by 2-tailed Student’s t test or one-way ANOVA. N = 3