Fig. 4

PITPNC1 exhibited a strong positive correlation with the expression of FASN and CD155. A Analysis results of fat acid metabolism-associated pathways. “High” represents the group with the high expression of PITPNC1, and “low” represents the group with the low. B Immunohistochemical comparison of FASN and CD155 expression levels in radiosensitive and unsensitive rectal cancer tissues. N = 7. C Western blotting employed to assess the expression levels of FASN and CD155 in tumor cell lines. N = 3. D Immunohistochemical analysis of protein expression levels of Ki67, PITPNC1, FASN, and CD155 in mouse tissues. N = 5. E Immunofluorescence co-localization of PITPNC1 and FASN proteins in cells and tissues. F CO-IP experiment performed on HT29 and SW480 cell lines to examine the interaction between PITPNC1 and FASN. G The expression levels of FASN and CD155 assessed by western blotting after knocking down PITPNC1 in tumor cell lines. N = 3. Data indicate the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, by 2-tailed Student’s t test or one-way ANOVA