Fig. 3

Cells and tumors displaying radioresistance evaded immune eradication by suppressing the immune system. A Analysis of the immune-checkpoint-related genes. “High” represented the group with the high expression of PITPNC1, and “low” represented the group with the low. B Flow cytometry was performed to analyze the changes in the proportion of CD8⁺ T cells after co-culturing PBMCs with tumor cells. N = 3. C The alterations in the proportion of IFN-γ⁺ CD8⁺ T cells assessed by flow cytometry after co-culturing PBMCs with tumor cells. N = 3. D The flow cytometry analysis of the apoptosis rate of tumor cells after co-culture. N = 3.E Conceptual diagram of the constructed animal model. F Graph depicting the weight changes in the animal model. G Statistical chart showing the volume of subcutaneous tumors in mice. N = 5. H Immunohistochemical analysis of protein expression levels of CD8 in mouse tissues. N = 5. I Immunofluorescence analysis of the proportion of IFN-γ⁺ CD8⁺ T cells. N = 5. J Flow cytometry analysis of the proportion of CD8⁺ T cells in the tumor tissues. N = 5. WT Wild-type group, RR Radioresistant group. Data indicate the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, by 2-tailed Student’s t test or one-way ANOVA