Fig. 2

Expression of PITPNC1 and its association with radioresistance in rectal cancer. A Differential gene heatmap analysis of expression chip data from 16 patients with rectal cancer. This figure displayed the top 55 up-regulated genes and the top 30 down-regulated genes. The color gradient in the heatmap ranged from blue to red, indicating low to high expression levels, respectively. B Volcano plot: The plot was generated using fold change values and p-adjust. Red dots represent upregulated genes, while blue dots represent downregulated genes. C Comparing the expression levels of the PITPNC1 in tumor tissues. N = 8. D A comparison of PITPNC1 expression levels in sensitive and unsensitive rectal cancer tissues was conducted using immunohistochemistry. N = 7. E After 6 Gy irradiation, the expression levels of PITPNC1, Bcl-2, Bax, and Cleaved caspase-3 were detected using western blotting. N = 3. F Flow cytometry analysis of cell apoptosis rates treated with 6 Gy irradiation. N = 3. G Cell viability assessed by CCK-8 after 6 Gy irradiation. N = 3. H–I F and G without 6 Gy irradiation. N = 3. RES Resistant, CR Complete Remission, C Control, RR Radioresistance. Data indicate the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, by 2-tailed Student’s t test or one-way ANOVA