Fig. 1

Establishment and validation of radioresistant cell strains. A Schematic illustration of radioresistant cell strains construction. B Flow cytometry analysis of apoptosis rate in HT29, SW480, and MC38 radioresistant cell strains and radiosensitive groups after 6 Gy radiotherapy. C Flow cytometry analysis of cell cycle changes between the two groups after 6 Gy radiotherapy. D CCK-8 assay to determine cell inhibition rate of the two groups. E Evaluation of colony formation by survival curves. Data indicate the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, by 2-tailed Student’s t test or one-way ANOVA. N = 3