Fig. 4

Tirzepatide upregulates the expression of neurodifferentiation markers: A Western Blot analysis of AKT (left panel) and p-AKT (central panel). Ratio between the phosphorylated and total forms of AKT (each one normalized with its vinculin), B MAP2, C GAP43 and D AGBL4 mRNA expression levels in SHSY5Y cells (left panel). The fold increase of mRNA expression compared with NG was calculated using the 2^−ΔΔCt method. Western Blot analysis of MAP2 (B), GAP43 (C) and AGBL4 (D) (right panel). The histograms show the densitometric analysis of 3 separate experiments representing the relative expression being NG value set as 1. Data are mean ± SEM. *p < vs NG; **p < vs HG