Fig. 3
From: Tirzepatide prevents neurodegeneration through multiple molecular pathways
Tirzepatide induces downregulation of apoptosis markers in neuronal cells: A Bcl-2 and B BAX mRNA expression levels in SHSY5Y cells (left panel). The fold increase of mRNA expression compared with NG was calculated using the 2−ΔΔCt method. Western Blot analysis of Bcl-2 (A) and BAX (B) (right panel). The histograms show the densitometric analysis of 3 separate experiments representing the relative expression being NG value set as 1. C BAX/Bcl-2 protein expression ratio. Data are mean ± SEM. *p < vs NG; **p < vs HG