Fig. 4
From: Systematic integration of molecular and clinical approaches in HCV-induced hepatocellular carcinoma
Invasion and migration assay of regulated RCN1 in L-HCV in vitro. A Expression of RCN1 in L-HCV cells transfected with pCMV6 empty vector, pCMV6-RCN1 (overexpressing RCN1), shNC (short hairpin normal control), and shRCN1 (short hairpin RCN1). Representative images and graphical display of cell invasion in L-HCV cells in vitro transwell assay: B, C Quantitative and qualitative analysis of L-HCV cells transfected with pCMV6 empty vector, pCMV6-RCN1, shNC, and shRCN1. D, E Quantitative and qualitative analysis of L-HCV cell migration in an in vitro wound healing assay in regulated RCN1. Time-lapse microscopy images of wound closure in L-HCV cells. Experiments were conducted in triplicates. Statistical significance p < 0.05*, p < 0.01**