Fig. 3

The immunosuppressive phenotype of obese CRC-derived myeloid cells. A UMAP plots of the myeloid cell subsets, colored by cell subsets. B Tissue prevalence of each myeloid cell subset estimated by Ro/e score, in which Ro/e denotes the ratio of observed to expected cell number. C The expression of the marker genes of the myeloid cell subsets. The pct.exp reflects the percentage of cells expressing the gene at non-zero levels. The Average expression reflects the averaged log-normalized expression. D Log-normalized expression of selected marker genes in myeloid cells. E Dot plots showing the metabolic activity analysis of three lipid-associated macrophages (LAMs) subsets by scMetabolism. The circle size and color darkness both represent the scaled metabolic score. F Violin plots showing the metabolic score of metabolic pathways in three LAMs subsets. Comparisons were performed by unpaired two-tailed Student’s t-test. Significance levels are expressed as *P < 0.05, **P < 0.01 and ***P < 0.001. G Violin plots showing MHC class II molecules expression of DC from obese CRC and non-obese CRC samples. H Violin plots showing the antigen presentation score in four DCs subsets. Comparisons were performed by unpaired two-tailed Student’s t-test. Significance levels are expressed as *P < 0.05, **P < 0.01 and ***P < 0.001