Fig. 5
Experimental validation of selected KDTs in rd10 mice, murine model of Retinitis Pigmentosa. Normalized gene expression profiles of selected KDTs from postnatal day (P) 15–60 with the observed peak of rod degeneration at P18 (A). Gene expression of K+/Cl− co-transporter 2 (Slc12a5) (B), alpha 1 and epsilon subunits of GABA A receptor (Gabra1 and Gabre) (C, D), glutamate ionotropic receptor NMDA type subunit 1 (Grin1) (E), and glycine receptor alpha 2 subunit (Glra2) (F), retinal GABA concentration in retinal extracts (G, the protein content of KCC2 (H) and GABARα1 (I) and NR1 (J) with representative western blots at P23 in control and rd10 mice. The unpaired t-test was used to compare control “C” and rd10 mice. *p < 0.05; **p < 0.01; ***p < 0.001. All data were presented as the mean ± standard error of the mean (SEM). We analyzed at least eight retinas for each group and age