Fig. 7
The FBXW7/CHD4 axis regulates the Wnt/β-catenin pathway in TNBC cells. A Enrichment plots from GSEA to examine the correlation between CHD4 expression and multiple cancer-related pathways based on TCGA-BRCA-TNBC datasets. B Pearson’s correlation analysis of the relationship between β-catenin expression and CHD4 expression was performed. (r = 0.5131, p < 0.0001). C The TOP/FOP assay was applied to measure the activity of the Wnt signaling pathway in cells transfected with shControl or shCHD4. D, E Immunofluorescence assay examining the nuclear translocation of β-catenin upon treatment with the indicated shRNA. Cell nuclei are stained with DAPI dye (blue). β-Catenin fluorescence intensity was analyzed and quantified by ImageJ (right panel) (200 ×). F, G Western blot analysis was used to examine the β-catenin protein level in the nuclear fraction (nucleus). The quantification of β-catenin protein levels was normalized to that of p84 (nucleus)