Fig. 6

FBW7 serves as an E3 ubiquitin ligase for the CHD4 protein. A, B Western blot analysis of whole cell lysates (WCLs) and immunoprecipitates derived from HEK-293 T cells transfected with the indicated plasmids for 24 h; cells were treated with 20 μM MG132 for an additional 5 h before harvesting. The ubiquitination status of endogenous CHD4 protein or Flag-tag protein was determined by immunoprecipitation (IP). GAPDH was used as an equal loading control