Fig. 2

FBXW7 suppresses cell proliferation, cell cycle progression, apoptosis, and EMT in TNBC cells. A Western blot and qRT‒PCR analysis of FBXW7 expression following FBXW7 knockdown in MD-MBA-231 cells and FBXW7 overexpression in HCC1937 cells. ***p < 0.0001. B Colony formation assays were performed to measure the proliferation ability of the transfected TNBC cells. C The levels of EMT marker proteins in the transfected TNBC cells were detected using Western blot analysis. D Transwell assays were performed to determine the migration abilities after FBXW7 knockdown and overexpression in MD-MBA-231 and HCC1937 cells, respectively. E, F FACS assays were performed on the transfected TNBC cells to analyze the cell cycle and apoptosis