Fig. 1

Low expression of FBXW7 in TNBC patients is associated with a poor prognosis. A Representative images of immunohistochemical staining of FBXW7 in TNBC tissues and corresponding adjacent noncancerous tissues using TMA tissue sections (n = 80 TNBC). B Quantitative values for IHC staining of FBXW7 between tumor tissues and adjacent nontumor normal tissues are presented in the histogram. Statistical analysis was performed by two-tailed paired Student’s t test (p = 0.0012). C, D Western blot and qRT‒PCR analysis of FBXW7 expression in mammary epithelial cell lines (MCF-10A), two non-TNBC cell lines (MCF-7 and T47-D) and three TNBC cell lines (HCC1937, BT549 and MD-MBA-231). E Kaplan–Meier survival curves for OS of TNBC patients in the high FBXW7 and low FBXW7 groups. F, G Kaplan‒Meier survival curves of DMFS and DFS based on FBXW7 expression using the bc-GenExMiner online tool (http://bcgenex.ico.unicancer.fr/BC-GEM/GEM-Accueil.php?js=1); HR values are shown