Fig. 5

Spatial colocalization of macrophages, hepatocytes, as well as MIF, CD74, and CD44. A Mapping of the enrichment scores of macrophages and hepatocytes onto a single-cell spatial transcriptome map, reflecting their infiltration in both tumor and normal tissues, by using the Addmodulescore function. B One-way analysis of variance (ANOVA) was performed to test the infiltration scores of macrophages in the four spatial transcriptome atlases. Significance is denoted as follows: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. C–F Spatial colocalization of MIF with CD44 and CD74 in the Control1 group (C), Control2 group (D), Tumor1 group (E), and Tumor2 group (F). Exp means the relative expression of the ligand and the receptor; colocalization means the interaction strength