Fig. 6

miR-218-5p overexpression suppresses IFN-I and inflammatory responses in BV2 cells treated with MPP + conditioned media. A A schematic diagram showing treatment of conditioned medium (CM) from MPP⁺ stimulated SH-SY5Y cells into BV2 cells. B miR-218-5p expression in BV2 cells treated with PBS-conditioned media (PBS-CM) and MPP⁺-conditioned media (MPP⁺-CM) is shown by qPCR analysis. N = 3 per group. C Effective miR-218-5p overexpression in the BV2 cells transfected with miR-218-5p mimic was verified by qPCR analysis. N = 6 per group. D–H Ifnb1 (D), Irf7 (E), Il6 (F), Il1b (G) and Tnf (H) mRNA expression in BV2 cells transfected with miR-218-5p mimic and stimulated with or without MPP⁺-CM is shown by qPCR analysis. N = 3 per group. I, J DDX41 and IL-1β protein levels in BV2 cells transfected with miR-218-5p mimic and stimulated with or without MPP⁺-CM by western blot (I) and quantitative analysis (J) are shown. N = 3 per group. Data are shown as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001