Fig. 5

Ddx41 is a target gene of miR-218-5p. A The binding sites of mmu-miR-218-5p with Ddx41 3′UTR. B, C DDX41 expression in the SN by western blot (B) and quantitative analysis (C) of NC Control, NC MPTP, miR-218 Control and miR-218 MPTP mice. N = 3 per group. D PmirGLO vectors including wildtype (WT) or mutated (MUT) 3′UTR of Ddx41 were co-transfected with miR-218-5p mimic or mimic NC in HEK293T cells. Luciferase activity was measured. N = 4 per group. Data are shown as the mean ± SEM. Significance was tested by two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001