Fig. 2

miR-218-5p overexpression inhibits microglial inflammatory and IFN-I responses in the SN in MPTP-induced mice. A Representative confocal images and 3D reconstructed images staining of IBA1 (red) and CD68 (green) in the SN of NC Control, NC MPTP, miR-218 Control and miR-218 MPTP mice. Scale bar, 25 μm. B Quantification of IBA1⁺ cells, IBA1⁺ cell volume and the ratio of CD68⁺IBA1⁺ puncta volume to IBA1⁺ cell volume in the SN of NC Control, NC MPTP, miR-218 Control and miR-218 MPTP mice. N = 4 per group. C Representative confocal images staining of IBA1 (red) and IRF7 (green) in the SN of NC Control, NC MPTP, miR-218 Control and miR-218 MPTP mice. Scale bar, 25 μm. D Quantification of the average intensity of IRF7 protein in IBA1⁺ cells in the SN of NC Control, NC MPTP, miR-218 Control and miR-218 MPTP mice. Data are shown as the mean ± SEM. Significance was tested by two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001