Fig. 5

FO upregulated SIRT1 suppressed NLRP3 inflammation activation. A mRECs were treated with with FO (60 μg/ml) for an additional 4 h, and then treated with Ang II (100 nM) for 24 h. 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) staining of ROS production in cells (left), the quantification of ROS intensity (n = 6). B qPCR analyses of Sirt1, Nlrp3 and Il1b mRNA in mRECs (n = 6). C The expression of SIRT1, NLRP3, IL-1β and IL-1β p17 protein levels. D The quantification of protein expression (n = 4). *P < 0.05, **P < 0.01, ***P < 0.0001 vs control; ^#P < 0.05, ^##P < 0.01 vs Ang II group