Fig. 4

Effect of overexpression of SIRT1 on NLRP3 inflammasome. A Mouse retinal vascular endothelial cells (mRECs) were treated with SRT1720 (0.5 μM) for an additional 1 h and then treated with Ang II (100 nM) for 24 h. qPCR analyses of Nlrp3 and Il1b mRNA in mRECs (n = 6). B The expression of NLRP3, IL-1β and IL-1β p17 protein levels. C The quantification of protein expression (n = 4). (D) DCFH-DA staining of ROS production in cells (left), the quantification of ROS intensity (n = 6). **P < 0.01, ***P < 0.0001 vs control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.0001 vs Ang II group