Fig. 6

P4HA1 promotes glycolysis in HUVECs by regulating FBP1 expression. A Volcano plot showing differentially expressed genes in HUVECs overexpressing P4HA1 (blue, downregulated genes; red, upregulated genes; gray, genes not significantly changed). B RT-PCR analysis of relative mRNA levels of FBP1 and P4HA1 in HUVECs after infection with Ad-P4HA1 or Ad-NC for 24 h (n = 3). C RT-PCR analysis of relative mRNA levels of FBP1 and P4HA1 in HUVECs after infection with Ad-shNC or Ad-shP4HA1 for 24 h (n = 3). D Western blotting analysis of FBP1 and P4HA1 protein levels in HUVECs after infection with Ad-P4HA1, Ad-NC, Ad-shNC, or Ad-shP4HA1 for 24 h. Western blotting results were statically analyzed (n = 3). E–G After HUVECs were infected with Ad-NC, Ad-P4HA1 alone, or Ad-P4HA1 and Ad-FBP1 for 24 h, E mRNA levels of PFKFB3, ALDOA, HK2, LDHA, GLUT1, P4HA1, and FBP1, F lactate production levels, and G glucose uptake levels were measured and analyzed (n = 3). H ECAR of HUVECs infected with Ad-NC, Ad-P4HA1 alone, or Ad-P4HA1 and Ad-FBP1 was measured using a Seahorse XF96 Flux Analyzer. Glycolysis and glycolytic capabilities were analyzed statistically (n = 3). Data in B, C were analyzed using the Student’s t-test. Data in D–H were analyzed by one-way ANOVA followed by Bonferroni post hoc test. *p < 0.05. NC negative control