Fig. 3

Interferon-stimulated B cells are enriched in MIBC. A tSNE plots of B cells in MIBC; n = 7; NMIBC, n = 3; normal, n = 9; PBMC, n = 4; lymph nodes, n = 3. Each cluster is shown in a different color. B The bar plots depict the mean cell count of each B-cell subtype in each sample on the left. In the middle, the bar plots illustrate the proportion of each B-cell subtype in each sample. On the right, a box plot displays the proportion of B-ISG15 in each sample.; MIBC, n = 7; NMIBC, n = 3; Normal, n = 9; PBMC, n = 4; lymph nodes, n = 3. C Heatmap shows the GSEA score of a set of hallmark genes enriched in B-cell subtypes. D Heatmap showing the activity of SCENIC transcription factor regulons in each B-cell subtype. E Expression levels of STAT1 and IRF9 marker genes illustrated in tSNE plots in each B-cell subtype. F The violin plots depict the expression patterns of IFN-γ genes, colors as in (A)